Coding

Part:BBa_K1977003:Experience

Designed by: Amritpal Singh   Group: iGEM16_Westminster_UoW   (2016-10-08)


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Heme F is known to be a precursor to protoporphyrin IX, which gives rise to hemes. It is known to be toxic to bacteria as Protoporphyrinogen IX is a photosentisiser. Upon light UV activation, ROS are released which causes damage to the cell. Therefore we decided to characterise the extent of toxicity using IPTG to suppress the activity of the natural Lac I repressor in XL-1 blue competent cells.


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Content

IPTG concentration (MM)

Absorbance

1

0

1.471

2

0.2

1.231

3

0.4

1.159

4

0.6

0.967

5

0.8

0.678

We found that increasing IPTG concentrations correlated to a decreased absorbance. This leads us to believe that Heme F is toxic to bacterial cells even at low concentrations of IPTG.

Applications of BBa_K1977003

Westminster 2017 characterised the effect of high expression on its toxicity to the host bacterial cell.

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